生态与农村环境学报 ›› 2015, Vol. 31 ›› Issue (3): 334-339.doi: 10.11934/j.issn.1673-4831.2015.03.010

• 自然保护与生态 • 上一篇    下一篇

蓝藻水华消亡对湖泊表层沉积物中氨氧化细菌丰度和群落结构的影响

黄睿,沈烽,罗娟,王司辰,唐启彤,徐慧敏,吴燕,赵大勇   

  1. 河海大学水文水资源与水利工程科学国家重点实验室
  • 收稿日期:2014-09-15 修回日期:2015-03-03 出版日期:2015-05-25 发布日期:2015-09-22
  • 通讯作者: 赵大勇 河海大学水文水资源与水利工程科学国家重点实验室 E-mail:dyzhao@hhu.edu.cn
  • 作者简介:黄睿(1989—),男,江苏泰兴人,博士生,主要研究方向为水生态环境保护与修复。E-mail:huangrui_sheng@sina.com
  • 基金资助:

    国家自然科学基金(41371098,43123001); 水文水资源与水利工程科学国家重点实验室专项经费(20145027312); 中国博士后科学基金(2014T70470,2014M561568); 江苏省博士后科研资助计划(1401093C); 河海大学水文水资源与水利工程科学国家重点实验室开放课题(2013491211)

Effects of Withering of Cyanobacteria Bloom on Abundance and Community Composition of Ammonia-Oxidizing Bacteria in Surface Lake Sediments

HUANG  Rui, SHEN  Feng, LUO  Juan, WANG  Si-Chen, TANG  Qi-Tong, XU  Hui-Min, WU  Yan, ZHAO  Da-Yong   

  1. State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering,Hohai University
  • Received:2014-09-15 Revised:2015-03-03 Online:2015-05-25 Published:2015-09-22
  • Contact: ZHAO Da-Yong State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering,Hohai University E-mail:dyzhao@hhu.edu.cn

摘要: 为探索蓝藻水华消亡对湖泊表层沉积物中氨氧化细菌(ammonia?oxidizing bacteria,AOB)的影响,设计了室内沉积物-水微宇宙模拟体系,体系中分别添加野外收集蓝藻和室内培养蓝藻,运用荧光定量PCR 和克隆建库等分子生物学方法分析氨氧化细菌的丰度、群落结构和多样性。结果表明:(1)添加蓝藻处理组的氨氧化细菌丰度和群落多样性高于未添加蓝藻的空白对照组;(2)属于亚硝化单胞菌属(Nitrosomonas)的N. oligotropha 类群在表层沉积物的氨氧化细菌中占有绝对优势,相对丰度达75%;(3)与空白对照组相比,添加蓝藻处理组的氨氧化细菌中新出现了N. communisNitrosospira 类群。添加蓝藻会提高沉积物中氨氧化细菌的丰度并增加其群落多样性。

关键词: 蓝藻, 氨氧化细菌, 克隆建库, 群落结构, 丰度

Abstract: A simulation system was constructed to investigate effect of withering of Cyanobacteria bloom on abundance and community compostition of ammonia-oxidizing bacteria (AOB) in surface sediments.The system consisted of three treatments, [i.e. (1) CK, (2) addition of lab-cultured Cyanobacteria and (3) addition of wild Cyanobacteria collected from a lake]. Abundance, community composition and diversity of AOB were analyzed with the real-time qPCR and molecular biological methods, like clone library, etc.. Results show that (1) Treatments 2 and 3 were much higher than Treatment 1 in abundance and community diversity of AOB; (2) N. oligotropha group of Nitrosomonas was the dominant AOB group, accounting for approximately 75% of the total AOB community in the surface sediments; and (3) Compared to Treatment 1, Treatments 2 and 3 were found to have some new AOB groups, such as N. communis and Nitrosospira. The findings indicate that N.oligotropha is absolutely the dominant group in all the treatments, and withering of Cyanobacteria bloom may improve AOB in the surface lake sediments in both abundance and diversity.

Key words: Cyanobacteria, ammonia-oxidizing bacteria, clone library, abundance and community composition, abundance

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